22 papers · ranked by Valyu relevance
Autumn T. LaPointe, Stefan D. Klimaj, Alison M. Kell
Hantaviruses are zoonotic, tri-segmented, negative-sense RNA viruses and a significant public health threat. Viral pathogenesis varies between host species, with rodent reservoir infection being asymptomatic and human infection resulting in severe, immune-mediated disease. Viral pathogenesis is highly dependent on…
Andrew C. Nelson, Benjamin Auch, Matthew Schomaker, Daryl M. Gohl + 11 more
The COVID-19 global pandemic is an unprecedented health emergency. Insufficient access to testing has hampered effective public health interventions and patient care management in a number of countries. Furthermore, the availability of regulatory-cleared reagents has challenged widespread implementation of testing. We…
Yang Zhang, Chunyang Dai, Huiyan Wang, Yong Gao + 7 more
'Yan Fang' 'Zuojun Shen' 'Lichang Chen' 'Zhaowu Chen' 'Xuejun Ma' 'Ming Li'] Background Coronavirus disease 2019 (COVID-19), caused by SARS-CoV-2, is posing a serious threat to global public health. Reverse transcriptase real-time quantitative polymerase chain reaction (qRT-PCR) is widely used as the gold standard for…
Anna Kristina Witte, Patrick Mester, Susanne Fister, Beate Süß + 2 more
'Martin Wagner' 'Peter Rossmanith'] Progressively more qPCR assays have been developed in recent years in numerous fields of application. These assays are routinely validated using calibration curves, but essential validation per se such as Poisson analysis is frequently neglected. However, validation is crucial for…
Peter B Dallas, Nicholas G Gottardo, Martin J Firth, Alex H Beesley + 6 more
'Katrin Hoffmann' 'Philippa A Terry' 'Joseph R Freitas' 'Joanne M Boag' 'Aaron J Cummings' 'Ursula R Kees'] Background The use of microarray technology to assess gene expression levels is now widespread in biology. The validation of microarray results using independent mRNA quantitation techniques remains a desirable…
Sushmita Sridhar, Sally Forrest, Iain Kean, Jamie Young + 11 more
The COVID-19 pandemic is expanding at an unprecedented rate. As a result, diagnostic services are stretched to their limit, and there is a clear need for the provision of additional diagnostic capacity. Academic laboratories, many of which are closed due to governmental lockdowns, may be in a position to support local…
Wenping Lian, Huifang Jin, Jingjing Cao, Xinyu Zhang + 8 more
'Shuai Zhao' 'Sujun Wu' 'Kailu Zou' 'Xinyun Zhang' 'Mingliang Zhang' 'Xiaoyong Zheng' 'Mengle Peng'] Background Tumor progression and distant metastasis are the main causes of deaths in colorectal cancer (CRC) patients, and the molecular mechanisms in CRC metastasis have not been completely discovered. Methods We…
Abhay Kumar Pathak, Sukhad Kural, Shweta Singh, Lalit Kumar + 4 more
The emergent role of nucleic acid-based biomarkers, such as microRNAs (miRNAs), long non-coding RNAs (lncRNAs), and messenger RNAs (mRNAs), is becoming increasingly prominent in the realms of disease diagnostics and risk assessment. Quantitative reverse transcription PCR (qRT-PCR) is the primary analytical method for…
Yann Karlen, Alan McNair, Sébastien Perseguers, Christian Mazza + 1 more
'Nicolas Mermod'] Background PCR has the potential to detect and precisely quantify specific DNA sequences, but it is not yet often used as a fully quantitative method. A number of data collection and processing strategies have been described for the implementation of quantitative PCR. However, they can be…
Jing Li, Kuo Zhang, Yanxi Han, Rongxue Peng + 4 more
'Guigao Lin' 'Siu-Kei Chow'] Title: ABSTRACT Chlamydia trachomatis (CT) is a significant sexually transmitted pathogen known to evoke severe complications, including infertility. Nucleic acid amplification tests (NAATs) are recommended by the World Health Organization to detect CT infection. Furthermore, the…
Shengwen Calvin Li, Kara J. Sparks, Leonard S. Sender
Allogenic stem-cell therapies benefit patients in the treatment of multiple diseases; however, the side effects of stem-cell therapies (SCT) derived from the concomitant use of immune suppression agents often include triggering infection diseases. Thus, analysis is required to improve the detection of pathogen…
David Gleerup, Matthijs Vynck, Lien Gysens, Cindy De Baere + 5 more
Digital Polymerase chain reaction (dPCR) enables precise and absolute quantification of nucleic acids by partitioning samples into thousands of individual PCR micro-reactions. While it minimizes the need for standard curves and enhances reproducibility compared to qPCR, thorough assay validation remains crucial. We…
Po‐E Li, Adán Myers y Gutiérrez, Karen W. Davenport, Mark Flynn + 7 more
'Bin Hu' 'Chien‐Chi Lo' 'Elais Player Jackson' 'Migun Shakya' 'Yan Xu' 'Jason Gans' 'Patrick Chain'] Summary: Polymerase chain reaction-based assays are the current gold standard for detecting and diagnosing SARS-CoV-2. However, as SARS-CoV-2 mutates, we need to constantly assess whether existing PCR-based assays will…
Sanghyuk Ko
Different types of diagnostics methods were introduced to the public due to the recent global pandemic. From the real-time reverse transcript polymerase chain reaction (RT-qPCR) to antigen lateral flow assay (LFA), these tests allow relatively accurate and rapid diagnosis of the infection with SARS-CoV-2 virus. Because…
Authors not listed
Large-scale de novo nucleic acid synthesis is a powerful tool enabling researchers to better understand and engineer biological systems. Fields ranging from genomics to nucleic acid therapeutics to synthetic biology make use of high-throughput experimental approaches requiring access to large pools or libraries of DNA…
Vincent Beguin, Jean Grétillat, Kornelija Kaminskaitė, Simonas Juzėnas + 14 more
Vincent Beguin1,\, Jean Grétillat2,\, Kornelija Kaminskaitė 3 , Simonas Juzenas 3 , Dainius Kirsnauskas 3 , Pierre-Yves Burgi 4 , Samuel Wenger 1 , Valentin Remonnay 1 , Silvia Angeloni 1 , Bart van der Schoot 5 , Augustin Cerveaux 5 , Thomas Heinis 6 , Renaldas Raisutis 7 , Martin Jost 8 , Lukas Zemaitis 3 , Ignas…
Authors not listed
The COVID-19 pandemic underscored the global need for rapid, sensitive, and multiplexed diagnostic assays for viral detection. RT-qPCR remains the standard for SARS-CoV-2 RNA detection, while antigen-based protein assays provide faster, though less sensitive, alternatives. Here, we present a novel diagnostic platform…
Authors not listed
Extreme weather events increasingly threaten coastal water quality, yet the mechanisms by which tropical cyclones impair microbial conditions remain poorly quantified. We develop a Large Language Model–Assisted Microbial Source Tracking (LAMST) framework to trace the origins of microbial threats—fecal indicator…
Emily E. Storey, Amr S. Helmy
Raman spectroscopy's capability to provide meaningful composition predictions is heavily reliant on a pre-processing step to remove insignificant spectral variation. This is crucial in biofluid analysis. Widespread adoption of diagnostics using Raman requires a robust model which can withstand routine spectra…
Paweł Domagała, Jolanta Hybiak, Violetta Sulżyc‐Bielicka, Cezary Cybulski + 2 more
'Cezary Cybulski' 'Janusz Ryś' 'W Domagała'] > Identifying targets for personalized targeted therapy is the pathologist's domain and a treasure. For decades, pathologists have had to learn, understand, adopt and implement many new laboratory techniques as they arrived on the scene. Pathologists successfully integrate…
Adán Andrés Ramírez Rojas, Cedric K. Brinkmann, Daniel Schindler
Golden Gate cloning has revolutionized synthetic biology. Its concept of modular, highly characterized libraries of parts that can be combined into higher order assemblies allows engineering principles to be applied to biological systems. The basic parts, typically stored in level 0 plasmids, are sequence validated by…
Yipeng Yin, Reed Arneson, Yinan Yuan, Shiyue Fang
The longest oligos that can be chemically synthesized using known methods are typically considered to be 200-mers. Here, we report direct synthesis of an 800-mer green fluorescent protein (GFP) gene and a 1,728-mer Φ29 DNA polymerase gene on an automated synthesizer. Key innovations that enabled the breakthrough…