25 papers · ranked by Valyu relevance
Wenfeng Tian, Riwang Chen, Liangyi Chen
Super-Resolution: An Odyssey in Harnessing Priors to Enhance Optical Microscopy Resolution Authors: Wenfeng Tian, Riwang Chen, Liangyi Chen As shown on the left side of [fig3](D), the distance between two peaks of a sine wave is called the wavelength λ, and the reciprocal of the wavelength is the frequency . A larger n…
Kirti Prakash, David Baddeley, Christian Eggeling, Reto Fiolka + 6 more
'Rainer Heintzmann' 'Suliana Manley' 'Aleksandra Radenovic' 'Hari Shroff' 'Carlas Smith' 'Lothar Schermelleh'] Title: ABSTRACT Super-resolution microscopy (SRM) has undeniable potential for scientific discovery, yet still presents many challenges that hinder its widespread adoption, including technical trade-offs…
Baptiste Gault, Michael P. Moody, Frédéric De Geuser, A. La Fontaine + 3 more
'Leigh T. Stephenson' 'Daniel Haley' 'Simon P. Ringer'] This article addresses gaps in definitions and a lack of standard measurement techniques to assess the spatial resolution in atom probe tomography. This resolution is known to be anisotropic, being better in the depth than laterally. Generally the presence of…
Tatiana Latychevskaia
This work presents an overview of the spatial resolution criteria in classical optics, digital optics and holography. Although the classical Abbe and Rayleigh resolution criteria have been thoroughly discussed in the literature, there are still several issues which still need to be addressed, for example the axial…
Anjum M. Karmali, Tom L. Blundell, Nicholas Furnham
Interpretation of low-resolution X-ray crystallographic data can prove to be a difficult task. The challenges faced in electron-density interpretation, the strategies that have been employed to overcome them and developments to automate the process are reviewed.
Yong Li, Chenguang Liu, Xiaoyu You, Jian Liu + 1 more
The three-dimensional (3D) displacement resolution of conventional visual measurement systems can only reach tens of microns in cases involving long measuring distances (2.5 m) and large fields of view (1.5 m × 1.5 m). Therefore, a stereo vision measurement technology based on confocal scanning is proposed herein. This…
Korak Kumar Ray, Colin D. Kinz-Thompson
The recent cryoEM resolution revolution has had a tremendous impact on our ability to investigate biomolecular structure and function. However, outstanding questions about the reliability of using a cryoEM-derived molecular model for interpreting experiments and building further hypotheses limit its full impact.…
David Kartte, Carsten Sachse
Resolution estimation by Fourier shell correlation (FSC) using half data sets is the standard method for map quality assessment in cryo-EM. Currently, the FSC method is largely used for refined cryo-EM maps in the context of single particle cryo-EM or subtomogram averaging. Here, we extended resolution estimation to…
Jane S. Richardson, Christopher J. Williams, Vincent B. Chen, Michael G. Prisant + 2 more
'Michael G. Prisant' 'David C. Richardson' 'R. Nicholls'] The explicit refinement of Ramachandran, rotamer and clash criteria at now-prevalent lower resolutions (2.5-4 Å) has made the current, traditional model validation at the Protein Data Bank nearly useless in this range, since quite poor structures can have…
Edward A. K. Cohen, Anish V. Abraham, Raimund J. Ober
We analyzed a number of well established algorithms and found that their algorithmic resolution limits can vary significantly (Figure 1). In fact, some of these algorithms are affected by algorithmic resolution well beyond the resolution limit that is predicted by Rayleigh’s criterion, which is around 250–300 nm for…
Johannes Emmert, Steven Wagner, Kyle J. Daun
Image based diagnostics are interpreted in the context of spatial resolution. The same is true for tomographic image reconstruction. Current empirically driven approaches to quantify spatial resolution [1] rely on a deterministic formulation based on point-spread functions which neglect the statistical prior…
Derek Mendez, James M. Holton, Artem Y. Lyubimov, Sabine Hollatz + 11 more
Artificial intelligence was used to characterize the diffraction in images from serial and rotation crystallography experiments. Forward simulations were used to train models to infer B factors, resolutions and the presence of crystal splitting from single diffraction images.
Aurel Radulescu, Noémi Kinga Székely, Stephan Polachowski, Marko Leyendecker + 12 more
'Marko Leyendecker' 'Matthias Amann' 'Johan Buitenhuis' 'Matthias Drochner' 'Ralf Engels' 'Romuald Hanslik' 'Günter Kemmerling' 'Peter Lindner' 'Aristeidis Papagiannopoulos' 'Vitaliy Pipich' 'Lutz Willner' 'Henrich Frielinghaus' 'Dieter Richter'] Using a double-disc chopper with a variable slit opening in concert with…
Vladimir Y. Lunin, Natalia L. Lunina
Uncertainties in atomic coordinates and loss of resolution blur atomic images on density maps. The resolution cutoff leads to an additional effect, the appearance of Fourier ripples. Ignoring these ripples makes it impossible to distinguish between the two sources of image blurring. However, separate determination of…
Authors not listed
The high lateral resolution and sensitivity of the NanoSIMS 50 and 50L series of dynamic SIMS instruments has enabled numerous scientific advances over the past 25 years. Here we report on the NanoSIMS-HR, the first major upgrade to the series, and analytical tests in a suite of sample types, including an aluminum…
Mathew J. Cherukara, Wonsuk Cha, Ross Harder
We demonstrate that the resolution of three-dimensional (3D) real-space images obtained from Bragg x-ray coherent diffraction measurements is direction dependent. We propose and demonstrate the effectiveness of a metric to determine the spatial resolution of images that accounts for the directional dependence. The…
John K. Eykelenboom, Marek Gierliński, Zuojun Yue, Nadia Hegarat + 4 more
When human cells enter mitosis, chromosomes undergo substantial changes in their organization – sister chromatids are resolved and chromosomes are compacted. However we still do not fully comprehend the timing and coordination of these events. To address these, we need to evaluate both sister chromatid resolution and…
Antonia Hamrick, Ofer Rog
Segregating a complete set of chromosomes into the gametes relies on exchanges of genetic material that occur during meiosis. It is only exchanges that form between the similar parental chromosomes (homologs), rather than between the identical sister chromatids, that enable correct chromosome segregation (57). Despite…
Guido Pauli, G. Joseph Ray, Anton Bzhelyansky, Birgit Jaki + 18 more
Classical 1D 1H NMR spectra are prototypic for NMR spectroscopy in that they represent a wealth of chemical information encoded into convoluted graphs or patterns that contain complex features (aka multiplets), even for seemingly simple molecules. Accordingly, the utility of NMR depends on the theoretical and visual…
Alexandre G. Urzhumtsev, Vladimir Y. Lunin
Refinement of macromolecular atomic models versus experimental maps in cryo-electron microscopy and crystallography is a critical step in structure solution. For an appropriate comparison, model maps should mimic imperfections of the experimental ones, mainly atomic disorder and its limited resolution, often…
Yide Zhang, Ufuk Yilmaz, Liam O’Faolain, Bernhard Lendl + 1 more
Atomic force microscopy-infrared spectroscopy (AFM-IR) is a photothermal scanning probe technique that combines nanoscale spatial resolution with the chemical analysis capability of mid-infrared spectroscopy. Using this hybrid technique, chemical identification down to the single molecule level has been demonstrated.…
Niko Vlahakis, Songrong Qu, Logan Richards, Lygia Silva deMoraes + 3 more
Electron counting helped realize the resolution revolution in single particle cryoEM and is now accelerating the determination of MicroED/3DED structures. Its advantages are best demonstrated by new direct electron detectors capable of fast (kilohertz) event-based electron counting (EBEC). This strategy minimizes the…
Authors not listed
The demand for high-resolution imaging of nanomaterials continues to grow across disciplines. However, conventional support films for transmission and scanning transmission electron microscopy (TEM/STEM) are often limited by low beam resistance, suboptimal resolution, toxicity concerns, and high production costs. This…
Amin Ghodousian, Mohammad Sedigh Chopannavaz
bipolar fuzzy relational equalities defined with continuous t-norms Authors: ['Amin Ghodousian' 'Mohammad Sedigh Chopannavaz'] This paper, in the first step, develops the system of bipolar fuzzy relational equations (FRE) to the most general case where the bipolar FREs are defined by an arbitrary continuous t-norm.…
Alžbeta Kubincová, Sereina Riniker, Philippe Herny Hünenberger
The Adaptive Solvent-Scaling (AdSoS) scheme [J. Chem. Phys. 155 (2021) 094107] is an adaptive-resolution approach for performing simulations of a solute embedded in a fine- grained (FG) solvent region surrounded by a coarse-grained (CG) solvent region, with a continuous FG↔CG switching of the solvent resolution across…