10 papers · ranked by Valyu relevance
Yuanmo Wang, Ajay Pradhan, Pankaj Gupta, Jörg Hanrieder + 2 more
Acetylcholine (ACh) is a critical neurotransmitter influencing various neurophysiological functions. Despite its significance, there is a lack of quantitative methods with adequate spatiotemporal resolution for recording single exocytotic efflux of ACh. In this study, we present an ultrafast amperometric ACh biosensor…
Afsaneh Taheri Kal-Koshvandi
We present the first light-free, genetically minimal platform for spatiotemporally resolved proximity labeling across five neuronal compartments—synaptic, nuclear, endosomal, mitochondrial, and activity-dependent microdomains. A covalent "catalyst–luciferase sandwich" architecture site-specifically conjugates five…
Authors not listed
Acetylcholine (ACh) is a key neurotransmitter involved in cognitive function, motor control, and synaptic modulation, yet its electrochemical inactivity and the rapid kinetics of exocytosis have hindered real-time quantal detection. Previous micrometer-scale enzymatic ACh biosensors enabled sub-millisecond…
Authors not listed
Researching the EEEV virus is challenging due to the virus's high lethality and significant risk. This study employed bioinformatics methods for domain search to investigate the pathogenesis and infection process of the EEEV virus P protein. The results demonstrated that the EEEV virus P protein contains domains that…
Farzaneh Asadpour, Xinwei Zhang, Mohammad Mazloum-Ardakani, Maysam Mirzaei + 2 more
We used liposomes loaded with different monoamines, dopamine (DA) and serotonin (5-HT), to simulate vesicular release and to monitor the dynamics of chemical release from isolated vesicles during vesicle impact electrochemical cytometry (VIEC). The release of DA from liposomes presents a longer release time compared to…
Authors not listed
The GluK1 receptor, an ionotropic glutamate receptor, operates as a ligand-gated ion channel. Opioids stand as the most potent pain-relieving medications currently prescribed in the medical field. Inhibiting this receptor is anticipated to result in a reduction of dopamine release in the Nucleus Accumbens, effectively…
Spencer Kim, Dylan Gray, Michelle Shanguhyia, Rachel Steinhardt
Recreating the signaling profile a chemical synapse to analyze serotonin receptor activation is a challenge. This is due in part to the kinetics of the synapse, where neurotransmitters are rapidly released and quickly cleared by active reuptake machinery. One strategy to produce a rapid rise in a bio-orthogonally…
wenzhong liu, hualan li
Infection with the Zika virus results in severe neurological disease in adults or congenital Zika syndrome in newborns. We employed the domain search strategy to study the Zika virus glycoprotein E in this work. The results revealed that immature E contains a NGF domain…
Authors not listed
Exosome-based therapies have emerged as a promising frontier in the field of regenerative medicine, particularly for neural repair. Exosomes, which are microvesicles derived from mesenchymal stem cells (MSCs), neural stem cells (NSCs), and induced pluripotent stem cells (iPSCs), exhibit significant regenerative…
Rumiana Tenchov, Janet Sasso, Qiongqiong Angela Zhou
Alzheimer's disease (AD) is a progressive neurodegenerative disorder characterized by cognitive decline, memory loss, and impaired daily functioning. The pathology of AD is marked by the accumulation of amyloid-beta plaques and tau protein tangles in brain, along with neuroinflammation and synaptic dysfunction. Genetic…