25 papers · ranked by Valyu relevance
Ruijie Cao, Tong Jin, Fengyuan Xin, Yiwei Hou + 16 more
Three-dimensional (3D) imaging represents the development of next generation of fluorescence microscopy. However, routine axial down-sampling makes isotropic resolution unrealistic. Here, we propose DeepUI, a physical zero-shot framework designed to achieve isotropic 3D fluorescence images from a low axial sampling…
Jacob R. Lamb, Conor McFadden, Reto Fiolka, James D. Manton
In oblique plane microscopy, a remote refocussing system is used to create an aberration-free 3D image of the object, from which an inclined plane is sampled using a tilted imaging system. As a result, unlike in conventional microscopes, the point spread function (PSF) and optical transfer function (OTF) are tilted…
Junzheng Peng, Manhong Yao, Zixin Cai, Xue Qiu + 3 more
'Shiping Li' 'Jingang Zhong'] Abstract: Digital two-dimensional (2D) spatial sampling devices (such as charge-coupled device) have been widely used in various imaging systems, especially in computational imaging systems. However, the undersampling of digital sampling devices is a problem that limits the resolution of…
Anthony DiSpirito, Daiwei Li, Tri Vu, Maomao Chen + 4 more
'Jianwen Luo' 'Roarke Horstmeyer' 'Junjie Yao'] Abstract—One primary technical challenge in photoacoustic microscopy (PAM) is the necessary compromise between spatial resolution and imaging speed. In this study, we propose a novel application of deep learning principles to reconstruct undersampled PAM images and…
Sulaimon Balogun, Andreas E. Vasdekis
Optical imaging systems are fundamentally constrained by a tradeoff between field of view (FoV) and spatial resolution. Long working-distance objectives, routinely used in biological imaging and especially in light-sheet microscopy, provide large FoV but reduced numerical aperture (NA) and magnification, broadening the…
Linjing Fang, Fred Monroe, Sammy Weiser Novak, Lyndsey Kirk + 12 more
Point scanning imaging systems (e.g. scanning electron or laser scanning confocal microscopes) are perhaps the most widely used tools for high resolution cellular and tissue imaging. Like all other imaging modalities, the resolution, speed, sample preservation, and signal-to-noise ratio (SNR) of point scanning systems…
Stephan Daetwyler, Reto Paul Fiolka
Light-sheet fluorescence microscopy has transformed our ability to visualize and quantitatively measure biological processes rapidly and over long time periods. In this review, we discuss current and future developments in light-sheet fluorescence microscopy that we expect to further expand its capabilities. This…
B. Francis, Manoj Mathew, Muthuvel Arigovindan
We consider the problem of reconstructing 2D images from randomly under-sampled confocal microscopy samples. The well known and widely celebrated total variation regularization, which is the ` 1 norm of derivatives, turns out to be unsuitable for this problem; it is unable to handle both noise and under-sampling…
Lyubov V. Amitonova, Johannes F. de Boer
For several centuries, far-field optical microscopy has remained a key instrument in many scientific disciplines, including physical, chemical, and biomedical research. Nonetheless, far-field imaging has many limitations: the spatial resolution is controlled by the diffraction of light, and the imaging speed follows…
Lyubov V. Amitonova, Johannes F. de Boer
For several centuries, far-field optical microscopy has remained a key instrument in many scientific disciplines including physical, chemical and biomedical research. Nonetheless, farfield imaging has many limitations: the spatial resolution is controlled by the diffraction of light and the imaging speed follows…
Mark A. Keppler, Sean P. O’Connor, Zachary A. Steelman, Xianglei Liu + 3 more
compressed streak imaging fluorescence microscopy Authors: ['Mark A. Keppler' 'Sean P. O’Connor' 'Zachary A. Steelman' 'Xianglei Liu' 'Jinyang Liang' 'Vladislav V. Yakovlev' 'Joel N. Bixler'] Compressed streak imaging (CSI), introduced in 2014, has proven to be a powerful imaging technology for recording ultrafast…
Mark A. Keppler, Sean P. O’Connor, Zachary A. Steelman, Xianglei Liu + 3 more
Compressed streak imaging (CSI) is a computational imaging strategy that can acquire video at over 150 trillion frames per second. Despite this achievement, CSI faces challenges in detecting subtle intensity fluctuations in slow-moving, continuously illuminated objects. This limitation, largely attributable to high…
Pooja Gaur, Beat Werner, Xue Feng, Samuel W. Fielden + 2 more
'Craig H. Meyer' 'William A. Grissom'] Background Volumetric thermometry with fine spatiotemporal resolution is desirable to monitor MR-guided focused ultrasound (MRgFUS) procedures in the brain, but requires some form of accelerated imaging. Accelerated MR temperature imaging methods have been developed that…
Christian T Stoeck, Robert Manka, Peter Boesiger, Sebastian Kozerke
Background CMR allows investigating cardiac contraction, rotation and torsion non-invasively by the use of tagging sequences. Three-dimensional tagging has been proposed to cover the whole-heart but data acquisition requires three consecutive breath holds and hence demands considerable patient cooperation. In this…
Haoran Wang, René Lachmann, Barbora Marsikova, Rainer Heintzmann + 1 more
State-of-the-art microscopy techniques enable the imaging of sub-diffraction barrier biological structures at the price of high-costs or lacking transparency. We try to reduce some of these barriers by presenting a super-resolution upgrade to our recently presented open-source optical toolbox UC2. Our new injection…
Tobias Wech, Angela Lemke, Debra Medway, Lee-Anne Stork + 4 more
'Craig A Lygate' 'Stefan Neubauer' 'Herbert Köstler' 'Jürgen E Schneider'] Purpose To combine global cardiac function imaging with compressed sensing (CS) in order to reduce scan time and to validate this technique in normal mouse hearts and in a murine model of chronic myocardial infarction. Materials and Methods To…
Changqing Su, Yanqin Chen, Zihan Lin, Zhen Cheng + 4 more
'Bo Xiong' 'Zhaofei Yu' 'Tiejun Huang'] Event cameras, an innovative bio-inspired sensor, differ from traditional cameras by sensing changes in intensity rather than directly perceiving intensity and recording these variations as a continuous stream of "events". The intensity reconstruction from these sparse events has…
Authors not listed
The self-organization of colloidal nanoparticles into complex structures, both in equilibrium and out-of-equilibrium, is a critical area in colloidal science with potential applications in creating new functional materials. While equilibrium assemblies yield thermodynamically stable and periodic structures…
Guoan Zheng, Xiaoze Ou, Changhuei Yang
Microscopy imaging systems with a very wide field-of-view (FOV) are highly sought in biomedical applications. In this paper, we report a wide FOV microscopy imaging system that uses a low-cost scanner and a closed-circuit-television (CCTV) lens. We show that such an imaging system is capable to capture a 10 mm 7.5 mm…
Hazen P. Babcock
This work explores the use of industrial grade CMOS cameras for single molecule localization microscopy (SMLM). We show that industrial grade CMOS cameras approach the performance of scientific grade CMOS cameras at a fraction of the cost. This makes it more economically feasible to construct high-performance imaging…
Authors not listed
The Single-probe is a multifunctional device that can be coupled to mass spectrometry (MS) for molecular analysis of microscale samples, such as single cells, tissue slices, and multicellular spheroids, under ambient conditions. In Single-probe single cell MS (SCMS) studies, this technique leverages direct sampling and…
Yong Wang, Xiushuo Zhang, Jing Xu, Xiangyu Sun + 9 more
'Hongsheng Li' 'Yanping Liu' 'Jingjing Tian' 'Xiaorui Hao' 'Xiaofei Kong' 'Zhiwei Wang' 'Jie Yang' 'Yuqing Su'] As a typical microscopic imaging technology, the emergence of the microscope has accelerated the pace of human exploration of the micro world. With the development of science and technology, microscopes have…
Authors not listed
Scanning emission-based microscopies, such as X-ray fluorescence (XRF) and energy-dispersive X-ray spectroscopy, offer nanometer-scale chemical maps, but suffer from long acquisition times and radiation damage. Lower-flux and shorter dwell time scans mitigate this problem, but the resulting signal loss can only…
Kanda Borgognoni, Bradley Guilliams, Rachel Cohen, Christopher Ackerson
A cloneable NanoParticle (cNP) is an inorganic nanoparticle that is synthesized by a protein. The protein determines the elemental composition, size, morphology, and other properties of the nanoparticle. Here, we describe the use of a cloneable Selenium NanoParticle (cSeNP) as a cloneable imaging contrast agent in…
Boris Louis, Sudipta Seth, Qingzhi An, Ran Ji + 3 more
Understanding the relationship between the active material's structure and function in operational devices is key for the rational engineering of the next generation of devices. However, this has proven elusive due to the invasiveness of structural characterization methods and their inability to penetrate the…