23 papers · ranked by Valyu relevance
Ruijie Cao, Tong Jin, Fengyuan Xin, Yiwei Hou + 16 more
Three-dimensional (3D) imaging represents the development of next generation of fluorescence microscopy. However, routine axial down-sampling makes isotropic resolution unrealistic. Here, we propose DeepUI, a physical zero-shot framework designed to achieve isotropic 3D fluorescence images from a low axial sampling…
Jacob R. Lamb, Conor McFadden, Reto Fiolka, James D. Manton
In oblique plane microscopy, a remote refocussing system is used to create an aberration-free 3D image of the object, from which an inclined plane is sampled using a tilted imaging system. As a result, unlike in conventional microscopes, the point spread function (PSF) and optical transfer function (OTF) are tilted…
Sulaimon Balogun, Andreas E. Vasdekis
Optical imaging systems are fundamentally constrained by a tradeoff between field of view (FoV) and spatial resolution. Long working-distance objectives, routinely used in biological imaging and especially in light-sheet microscopy, provide large FoV but reduced numerical aperture (NA) and magnification, broadening the…
Michael John Fanous, Paloma Casteleiro Costa, Çağatay Işıl, Luzhe Huang + 1 more
'Luzhe Huang' 'Aydogan Ozcan'] In recent years, the integration of deep learning techniques with biophotonic setups has opened new horizons in bioimaging. A compelling trend in this field involves deliberately compromising certain measurement metrics to engineer better bioimaging tools in terms of e.g., cost, speed…
Stephan Daetwyler, Reto Paul Fiolka
Light-sheet fluorescence microscopy has transformed our ability to visualize and quantitatively measure biological processes rapidly and over long time periods. In this review, we discuss current and future developments in light-sheet fluorescence microscopy that we expect to further expand its capabilities. This…
Saugat Kandel, Tao Zhou, Anakha V Babu, Zichao Wendy Di + 6 more
'Xuedan Ma' 'Martin Holt' 'Antonino Miceli' 'Charudatta Phatak' 'Mathew J. Cherukara'] The submitted manuscript has been created by UChicago Argonne, LLC, Operator of Argonne National Laboratory ("Argonne"). Argonne, a U.S. Department of Energy Office of Science laboratory, is operated under Contract No.…
Mark A. Keppler, Sean P. O’Connor, Zachary A. Steelman, Xianglei Liu + 3 more
compressed streak imaging fluorescence microscopy Authors: ['Mark A. Keppler' 'Sean P. O’Connor' 'Zachary A. Steelman' 'Xianglei Liu' 'Jinyang Liang' 'Vladislav V. Yakovlev' 'Joel N. Bixler'] Compressed streak imaging (CSI), introduced in 2014, has proven to be a powerful imaging technology for recording ultrafast…
Mark A. Keppler, Sean P. O’Connor, Zachary A. Steelman, Xianglei Liu + 3 more
Compressed streak imaging (CSI) is a computational imaging strategy that can acquire video at over 150 trillion frames per second. Despite this achievement, CSI faces challenges in detecting subtle intensity fluctuations in slow-moving, continuously illuminated objects. This limitation, largely attributable to high…
Tae Jun Jang, Chang Min Hyun
—This article presents a novel undersampled magnetic resonance imaging (MRI) technique that leverages the concept of Neural Radiance Field (NeRF). With radial undersampling, the corresponding imaging problem can be reformulated into an image modeling task from sparse-view rendered data; therefore, a high dimensional MR…
Authors not listed
Scanning ion conductance microscopy (SICM) offers non-contact, label-free imaging of live cells with nanometer-scale resolution. However, its conventional imaging mode is inherently slow due to repeated vertical scanning, limiting temporal resolution and causing inertial issues. Here, we present Scanning Counter Ion…
Hari Shroff, Ilaria Testa, Florian Jug, Suliana Manley
The proliferation of microscopy methods for live-cell imaging offers many new possibilities for users but can also be challenging to navigate. We focus here on computational methods that promise to boost live-cell fluorescence microscopy, where intra-cellular dynamics and cell viability constrain measurements…
Andrew Phair, Anastasia Fotaki, Lina Felsner, Thomas J. Fletcher + 3 more
Background Cardiovascular magnetic resonance (CMR) is an important imaging modality for the assessment and management of adult patients with congenital heart disease (CHD). However, conventional techniques for three-dimensional (3D) whole-heart acquisition involve long and unpredictable scan times and methods that…
David Hörl
Super-resolution microscopy enables the observation of cells at unprecedented detail but usually entails high light exposure and slow imaging. Thus, often only a few manually selected regions are imaged, limiting the ability to capture the distribution of quantitative features in a population of cells in an unbiased…
David Hörl
Super-resolution microscopy enables the observation of cells at unprecedented detail but usually entails high light exposure and slow imaging. Thus, often only a few manually selected regions are imaged, limiting the ability to capture the distribution of quantitative features in a population of cells in an unbiased…
Changqing Su, Yanqin Chen, Zihan Lin, Zhen Cheng + 4 more
'Bo Xiong' 'Zhaofei Yu' 'Tiejun Huang'] Event cameras, an innovative bio-inspired sensor, differ from traditional cameras by sensing changes in intensity rather than directly perceiving intensity and recording these variations as a continuous stream of "events". The intensity reconstruction from these sparse events has…
Authors not listed
The self-organization of colloidal nanoparticles into complex structures, both in equilibrium and out-of-equilibrium, is a critical area in colloidal science with potential applications in creating new functional materials. While equilibrium assemblies yield thermodynamically stable and periodic structures…
Sohaib Ayaz Qazi, Tamara Bianchessi, Federica Viola, Chiara Trenti + 3 more
Plots of average and maximum velocity in the thoracic aorta over the cardiac cycle for the prospectively undersampled acquisitions are shown in Figure [mrm70317-fig-0008]. Overall, there is good agreement between ground truth (fully sampled) and the average and maximum velocity for different acceleration factors…
Yasaman Kargar Gaz Kooh, Nathaniel Huebsch
COPYRIGHT © 2024 Kargar Gaz Kooh and Huebsch. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original…
Han Wang, Wenshu Wang, Xinzhu Xu, Meiqi Li + 1 more
Principle, Instrumentation, and Applications Authors: ['Han Wang' 'Wenshu Wang' 'Xinzhu Xu' 'Meiqi Li' 'Peng Xi'] Among super-resolution microscopy techniques, structured illumination microscopy (SIM) shows great advances of low phototoxicity, high speed, and excellent performance in long-term dynamic observation…
Alexander Carsten, Antonio Virgilio Failla, Martin Aepfelbacher
1## INTRODUCTION Fluorescence microscopy has played a prominent role in the natural and life sciences for many decades, as it generally does not require complex sample preparation protocols, but at the same time allows specific visualisation of almost all animate and inanimate matter. Furthermore, during the image…
Yong Wang, Xiushuo Zhang, Jing Xu, Xiangyu Sun + 9 more
'Hongsheng Li' 'Yanping Liu' 'Jingjing Tian' 'Xiaorui Hao' 'Xiaofei Kong' 'Zhiwei Wang' 'Jie Yang' 'Yuqing Su'] As a typical microscopic imaging technology, the emergence of the microscope has accelerated the pace of human exploration of the micro world. With the development of science and technology, microscopes have…
Ando C. Zehrer, Ana Martin-Villalba, Benedict Diederich, Helge Ewers
Fluorescence microscopy is a fundamental tool in the life sciences, but the availability of sophisticated equipment required to yield high-quality, quantitative data is a major bottleneck in data production in many laboratories worldwide. This problem has long been recognized and the abundancy of low-cost electronics…
Authors not listed
Scanning emission-based microscopies, such as X-ray fluorescence (XRF) and energy-dispersive X-ray spectroscopy, offer nanometer-scale chemical maps, but suffer from long acquisition times and radiation damage. Lower-flux and shorter dwell time scans mitigate this problem, but the resulting signal loss can only…